Abstract
Two novel species of the beer-spoilage Lactobacillus bacteria were isolated from the brewing environment. One species, of which LA-2 is a representative strain, is an obligately heterofermentative Lactobacillus sp. with a strong beer-spoilage ability. The other species, of which LA-6 is representative, is a facultatively heterofermentative Lactobacillus sp. with a weak beer-spoilage ability. The carbohydrate utilization pattern, ribotype, and DNA-DNA hybridization analysis revealed that these novel strains were not related at the species level to any Lactobacillus spp. examined in this study. However, the 16S ribosomal DNA sequence of LA-2 was found to exhibit 99.5% similarity with that of the L. collinoides gene, indicating that these species are related. We have also developed primer sets for the detection of the LA-2 and LA-6.
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