Abstract
Phytopythium iriomotense sp. nov. was isolated from river water of Iriomote Island, Okinawa Prefecture, Japan. The species can grow at 10–35 °C. The optimum temperature was 30 °C, with a radial growth of 24.3 mm per day. The species is morphologically characterized by globose to sub-globose sporangia with apical papillae, internally nested and internally extended proliferating sporangia, lateral hyphal swellings, compound sympodia, globose and smooth oogonia, amphigynous or crook-necked antheridial cells, oogonia with double oospores, aplerotic or plerotic oospores, and a chrysanthemum growth pattern on V8A. Phytopythium aichiense sp. nov. was isolated from water purification sludge in Aichi prefecture, Japan. The species can grow at 3–35 °C. The optimum temperature was 28 °C with a radial growth of 12.3 mm per day. P. aichiense is morphologically characterized by sub-globose, ovoid or limoniform sporangia with apical papillae, internally nested or internally extended proliferating sporangia, filamentous inflated or crook-necked antheridial cells, aplerotic oospores and a stellate growth pattern on V8A. Phylogenetic analyses based on the rDNA ITS region and combined phylogenetic trees based on the nuclear LSU rDNA and beta-tubulin gene, and the mitochondrial coxI and coxII genes, revealed that both species are genetically distinct from each other and from morphologically related species.
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