Two new SNPs within exon 1 of the porcine MYOD1 (MYF3) gene and their frequencies in chosen pig breeds and lines

Abstract

SummaryMyogenic factor 3 (MYF3, MYOD1) is a product of the MYOD1 gene, belonging to the MyoD gene family coding for the transcription factors which control the processes of myogenesis. Two fragments of exon 1 of the MYOD1 gene were amplified and sequenced. Two novel single nucleotide polymorphisms (SNPs), C489T and G566C, were identified in the fragment located between nucleotides 333 and 662. Transition C489T does not change the amino acid sequence (Leu), whereas transversion G566C leads to a replacement of arginine with proline at residue 76. Both SNPs were recognized with the BssSI restriction endonuclease. The distribution of alleles for both SNPs was tested in several pig breeds and lines raised in Poland (Polish Large White, Polish Landrace, Pietrain, Duroc, Zlotnicka Spotted, synthetic line L990). Duroc pigs proved homozygous at nucleotides 489 and 566 (haplotype T–C, respectively) and, compared with the remaining tested breeds (except for Zlotnicka Spotted), differed significantly in the frequency of alleles for both SNPs. The frequency of mutations C489T and G566C, observed among Zlotnicka Spotted pigs, was similar to that observed in the Duroc pigs and in the remaining breeds, respectively. As a result of the absence of polymorphism at both the nucleotides 489 and 566, Duroc pigs are not suitable for evaluating the effect of the SNPs identified in meat and carcass traits.