Two new polyhydroxylated steroidal glycosides from Paris polyphylla var. yunnanensis

Abstract

Chemical investigation of Paris polyphylla smith var. yunnanensis afforded two new polyhydroxylated steroidal glycosides, named Parisyunnanosides K and L ( 1 – 2 ), together with nine known ones. The chemical structures of the new compounds were elucidated by 1D, 2D NMR and HR-ESI-MS techniques, together with chemical methods. Parisyunnanosides K and L ( 1 – 2 ) are rare C 27 steroidal glycosides with two double bonds located at C-5, 6 and C-25, 26 of the aglycone, respectively. The cytotoxic activities of the isolated compounds were evaluated against Caco-2 cells by CCK-8 assay. Among them, compounds 5 – 11 exhibited potent cytotoxic activity with IC 50 values ranging from 1.10 to 14.14 μM compared with the positive control oxaliplatin (1.38 μM). Eleven steroidal saponins, including two previously unreported, were isolated from the rhizomes of P. polyphylla var. yunnanensis. The isolates 1 – 11 were evaluated for their cytotoxic activity in Caco-2 cells by using CCK-8 assay. • Two previously undescribed steroidal glycosides were characterized from Paris polyphylla . • The new compounds are rare C 27 steroidal glycosides with two double bonds of the aglycone. • Cytotoxic activities of the isolated compounds were tested on Caco-2 cancer cells.