Abstract
Two new bufadienolides, 3β,14β,16β-trihydroxy-5α-bufa-20,22-dienolide (1) and 14β-hydroxy-3β-[β-D-glucopyranosyl-(1→6)-(β-D-glucopyranosyl)oxy]-5α-bufa-20,22-dienolide (2), one new pregnane, 3β-hydroxypregna-5,16-diene-20-one-1β-yl sulfate (3), along with one known pregnane (4) were isolated from the dried roots and rhizomes of Helleborus thibetanus. Their structures were elucidated by the extensive use of 1D and 2D NMR experiments, together with IR and HRESIMS spectra and the results of enzymatic hydrolysis.
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