Abstract

Based on a survey of macro-fungi in northern and northeastern Thailand, nine samples collected in 2020 are identified as Amanita and introduced here as two new species, Amanita kalasinensis and A. ravicrocina. Typical macro- and microscopical characteristics indicate that both of these two species belong to Amanita section Amanita, but differ from other currently known species. Amanita kalasinensis is characterized by having a greyish yellow pileus covering with a conical to granuliform, yellowish white volval remnant; the presence of clamps; and a broadly ellipsoid to ellipsoid basidiospore. Amanita ravicrocina is characterized by having a brown to greyish orange pileus covering with a patchy, white volval remnant; a collar-like volval remnant on the stipe; and a subglobose to broadly ellipsoid basidiospore. Multi-gene phylogenetic analysis of partial nuclear rDNA internal transcribed spacer region (ITS), partial nuclear rDNA large subunit region (nrLSU), RNA polymerase II second largest subunit (RPB2), partial translation elongation factor 1-alpha (TEF1-α), and beta-tubulin gene (TUB) also revealed that positions of A. kalasinensis and A. ravicrocina are well-supported within A. section Amanita, but form distinct lineages and do not show any close relationship with any species. The detailed morphological features, line-drawing illustration, and comparison with morphological similar taxa are provided.

Highlights

  • Received: 27 December 2021Amanita Pers. is a widespread basidiomycetous genus comprising more than 600 species all over the world [1,2,3,4,5]

  • [13], and the remaining specimens were dried at 35–45 °C for at least twelve hours to prepare for the morphological material and later were deposited at the Herbarium of Biology Department (CMUB) and the Herbarium of Sustainable Development of Biological

  • The model HKY + I + G and K80 + G could not be implemented in result of maximum likelihood (RAxML), the GTR

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Summary

Introduction

Received: 27 December 2021Amanita Pers. is a widespread basidiomycetous genus comprising more than 600 species all over the world [1,2,3,4,5].

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