Abstract
Two new cycloartenol triterpene saponins, 7,8,16,17-tetrahydro-23R,24R-O-acetyl-hydroshengmanol-3-O-β-d-galactopyranoside (1), 16,17-didehydro-23R,24R-O-acetyl-hydroshengmanol-3-O-β-d-galactopyranoside (2), were isolated from the ethyl acetate soluble fraction of Cimicifuga simplex Wormsk. Their structures were established by detailed spectroscopic analysis, including extensive 2D NMR data. This is the first time to report 16,17-double bond type glycosidesaponin from Cimicifuga species. Their immunosuppressive activities were also carried out by ConA-stimulated T splenocyte proliferation assay in vitro.
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