Abstract
The details of exocytosis, the vital cell process of neuronal communication, are still under debate with two generally accepted scenarios. The first mode of release involves secretory vesicles distending into the cell membrane to release the complete vesicle contents. The second involves partial release of the vesicle content through an intermittent fusion pore, or an opened or partially distended fusion pore. Here we show that both full and partial release can be mimicked with a single large-scale cell model for exocytosis composed of material from blebbing cell plasma membrane. The apparent switching mechanism for determining the mode of release is demonstrated to be related to membrane tension that can be differentially induced during artificial exocytosis. These results suggest that the partial distension mode might correspond to an extended kiss-and-run mechanism of release from secretory cells, which has been proposed as a major pathway of exocytosis in neurons and neuroendocrine cells.
Highlights
The details of exocytosis, the vital cell process of neuronal communication, are still under debate with two generally accepted scenarios
These results suggest that the partial distension mode might correspond to an extended kiss-and-run mechanism of release from secretory cells, which has been proposed as a major pathway of exocytosis in neurons and neuroendocrine cells
We employ an artificial cell model previously developed using pure soy lipid preparations to study expansion of the fusion pore formed during exocytosis and the subsequent release of neurotransmitter[16]
Summary
The details of exocytosis, the vital cell process of neuronal communication, are still under debate with two generally accepted scenarios. The vesicle is inflated until the full distention of the nanotube into the giant liposome occurs resulting in the complete release of the vesicle content, mimicking the final stages of exocytosis Chemical release in these events can be studied using amperometry at a carbon fiber electrode placed against the giant liposome at the position of release. The partial distension mode demonstrates the ease with which the membrane forms these structures that mimic the recently presented, but still controversial, mode of extended kiss-and-run where the fusion pore is hypothesized to only transiently expand[8] These observations provide us with an advanced artificial system for studying both full and partial release of the vesicle content, possibly corresponding to the full and kiss-and-run exocytosis that is believed to occur in most secretory cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
