Abstract
Atomic force microscopy (AFM) and optical microscopy, in particular fluorescence microscopy, make a powerful combination in the study of biological samples. AFM is not subject to Abbe's resolution limit, and can generate images with a much higher resolution than light microscopy. However, as contrast is generated in response to the structural properties of the sample, it can be challenging to detect specific structures in a heterogeneous sample, such as a cell. By combining the two techniques, higher resolution structural information can be generated using AFM. Subsequent correlation with fluorescently labelled markers can provide information about the composition, and consequently the function, of the identified structures.
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