Abstract
Excessive UV exposures are commonly associated with adverse health effects, but proper amounts of UV are beneficial for people and essential in the natural production of Vitamin D 3 in skin. Two methods have been developed for direct evaluation of the Vitamin D synthetic capacity of sunlight (and artificial UV sources). The first one uses an in vitro model of Vitamin D 3 synthesis (ethanol solution of 7-dehydrocholesterol, 7-DHC), and concentration of previtamin D 3 accumulated during an UV exposure is determined using specially designed spectrophotometric analysis. The second method utilizes photoisomerization of provitamin D in nematic liquid crystalline (LC) matrix, and visual estimation of accumulated previtamin D becomes possible due to special design of a LC cell. This user-friendly method is appropriate for personal UV dosimetry and may have wide application in tanning saloons, in clinical dermatology and UV therapy.
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More From: The Journal of Steroid Biochemistry and Molecular Biology
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