Two Meridic Diets for Perillus bioculatus (Heteroptera: Pentatomidae), a Predator of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Abstract

Abstract Two artificial diets, devoid of insect components, were developed for the predator Perillus bioculatus (Fabricius). The diets based on chicken liver and tuna fish were designed to approach the nutritional characteristics of the primary insect prey, Leptinotarsa decemlineata (Say) eggs. P. bioculatus was reared on diet 2 for 11 consecutive generations without cumulative detrimental effects on its biology. Developmental time and preovipositional period were significantly longer, and egg viability, survival from egg to adult, and fecundity were significantly lower in P. bioculatus individuals reared on artificial diets than in those reared on L. decemlineata eggs. A significant reduction in developmental time, increase in survival from egg to adult, and reduction in length of the preovipositional period were observed after 11 generations of in vitro reared P. bioculatus on diet 2. These changes may indicate that the predators experience some degree of adaptation to the diet after several consecutive generations of in vitro rearing. No changes in egg viability were observed after 11 generations of in vitro rearing. Adult female weight was unaffected when reared on artificial diet, but a significant increase was observed after 11 generations of in vitro rearing. Females under all treatments weighed an average of 11.6 mg more than the males.