Abstract
Two new separations of folate derivatives by in situ paired-ion high-performance liquid chromatography are described. The first utilizes two C 18 phenyl columns and the second utilizes a C 8 radial compression cartridge. Folic acid, dihydrofolic acid, folinic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid and a decomposition product, p-aminobenzoylglutamic acid are separated. A comparison is made between these two methods and with the standard microbiological Lactobacillus casei assay for folates. The first method offers improved resolution and in situ sample clean-up. The second method accomplishes this in one-fifth the time. Both methods provide information not obtained by the microbiological assay.
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