Abstract
In this paper, two simple probes (L1 and L2) have been designed and synthesized for detection of ClO− with large Stokes shifts (about 200 nm). Both probes can react with ClO− in less than one min and display obvious changes in fluorescence, which showing potential ability of detection ClO− in real time. ES-MS analysis was utilized to confirm our design strategy and the possible detection mechanism. Owing to the delicate difference of structure of two probes, L2 has a significant fluorescence ratio response to ClO− and the fluorescence of L1 is quenched. L1 has a longer emission wavelength, better response characteristics and weaker cytotoxicity (IC50 28.1 μM) as compared with L2. The detection limit of L1 is even as low as 0.09 μM. What's more, it is successfully used in monitoring ClO− real water samples and in living cells.
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