Abstract
Flow profiles in a Z-type 0.6-μl micro flow-cell of an ultraviolet absorption detector for high-performance liquid chromatography were evaluated. The flow profile and band broadening in the flow-cell were calculated by finite-element analysis using a supercomputer (HITAC S-810). The flow profile was Poiseuille flow following the Golay equation at flow-rates less than 0.2 ml/min. However, at flow-rates above 0.2 ml/min the flow had two types of band broadening, depending on the form of the flow-cell and the connection between the flow-cell and the connecting tubing. The first type was band broadening independent of flow velocity, including mixing broadening at flow-rates from 0.2 to 1.0 ml/min. The second was mixture-type band broadening between laminar flow broadening and mixing broadening depending on the flow velocity, which gave a Gaussian profile containing a tailing peak, at flow-rates over 1.0 ml/min. A comparison of these results and the experimental results showed good agreement.
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