Two Clostridiumperfringens Type E Isolates in France.

Laure Diancourt,Jean Sautereau,Alexis Criscuolo,Michel Popoff

doi:10.3390/toxins11030138

Laure Diancourt, Jean Sautereau + Show 2 more

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https://doi.org/10.3390/toxins11030138

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Abstract

Clostridiumperfringens type E is a less frequently isolated C. perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C. perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and 515.17 from the stool of a 60-year-old woman, subsequent to food poisoning, which contained the plasmid pCPPB-1 with variant iota toxin and C. perfringens enterotoxin genes.

Highlights

Clostridium perfringens is a ubiquitous gram-positive, spore forming anaerobic bacterium which produces different toxins and is responsible for various diseases in man and animals such as gangrene, food poisoning, diarrhea, necrotic enteritis, and enterotoxemia
C. perfringens type E is a less frequently isolated type from clinical samples. This toxin type has been isolated from cases of diarrhea or hemorrhagic enteritis and sudden death in neonatal calves, mainly in the US, and more rarely in other animals such as chickens, lambs, goats, and cows [5,6,7,8,9,10,11,12,13]
Most C. perfringens type E strains have been found to contain the iota toxin genes on a plasmid flanked by the insertion sequence IS1151 and in close proximity to a silent cpe gene [17]

Summary

Introduction

Clostridium perfringens is a ubiquitous gram-positive, spore forming anaerobic bacterium which produces different toxins and is responsible for various diseases in man and animals such as gangrene, food poisoning, diarrhea, necrotic enteritis, and enterotoxemia. Based on the production of four toxins (alpha, beta, epsilon, and iota), C. perfringens was traditionally divided into five toxin types C. perfringens type E is characterized by the production of iota toxin. C. perfringens type E is a less frequently isolated type from clinical samples. This toxin type has been isolated from cases of diarrhea or hemorrhagic enteritis and sudden death in neonatal calves, mainly in the US, and more rarely in other animals such as chickens, lambs, goats, and cows [5,6,7,8,9,10,11,12,13]. In France, C. perfringens type E isolation has not been previously reported. We describe two recent type E isolates, 508.17 and 515.17

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