Abstract

Red blood cells are easily labelled with Cr 51 and blood plasma is readily tagged with I 131. However, the proximity of the principal gamma energies of these two isotopes complicates the use of differential pulse height analysis techniques for the measurement of doubly labelled blood. Instrumentation for accomplishing this procedure includes a 3 in. well-type scintillation counter operated with two single-channel differential pulse height analyzers. A method is presented for setting up two channels with the analyzers to obtain adequate separation of Cr 51 and I 131. A simple correction for the cross-channel activities is shown. The accuracy of the method is evaluated by the assay of mixed Cr 51 + I 131 sources prepared gravimetrically.

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