Abstract
Aquaporins (AQPs) facilitate the transport of water and small molecules across intrinsic membranes and play a critical role in abiotic stresses. In this study, 111, 54, and 56 candidate AQP genes were identified in Gossypium hirsutum (AD1), Gossypium arboreum (A2), and Gossypium raimondii (D5), respectively, and were further classified into five subfamilies, namely, plasma intrinsic protein (PIP), tonoplast intrinsic protein (TIP), nodulin 26-like intrinsic protein (NIP), small basic intrinsic protein (SIP), and uncategorized X intrinsic protein (XIP). Transcriptome analysis and quantitative real-time PCR (qRT-PCR) revealed some high-expression GhPIPs and GhTIPs (PIP and TIP genes in G. hirsutum, respectively) in drought and salt stresses. GhPIP2;7-silenced plants decreased in the chlorophyll content, superoxide dismutase (SOD) activity, and peroxidase (POD) activity comparing the mock control (empty-vector) under 400 mM NaCl treatment, which indicated a positive regulatory role of GhPIP2;7 in salt tolerance of cotton. The GhTIP2;1-silenced cotton plants were more sensitive to osmotic stress. GhTIP2;1-overexpressed plants exhibited less accumulation of H2O2 and malondialdehyde but higher proline content under osmotic stress. In summary, our study elucidates the positive regulatory roles of two GhAQPs (GhPIP2;7 and GhTIP2;1) in salt and osmotic stress responses, respectively, and provides a new gene resource for future research.
Highlights
Aquaporins (AQPs) are members of the major intrinsic protein (MIP) superfamily, contributing to the transport of water and small molecules across biological membranes in most organisms (Maurel et al, 2015)
Most GhAQPs that were belonging to the plasma intrinsic protein (PIP) subfamily were induced rapidly and continued to be upregulated at 3 h after salt or osmotic treatment
We focused on five cis-regulatory elements responding to abiotic stresses, including a cis-acting regulatory element essential for the anaerobic induction (ARE), a cis-acting element involved in low-temperature responsiveness (LTR), an MYB binding site involved in drought inducibility (MBS), a motif involved in differentiation of the palisade mesophyll cells (HD-Zip 1), and a wound-responsive element (WUN-motif) (Supplementary Table S3 and Supplementary Figure S2)
Summary
Aquaporins (AQPs) are members of the major intrinsic protein (MIP) superfamily, contributing to the transport of water and small molecules across biological membranes in most organisms (Maurel et al, 2015). AQPs are involved in the transportation of glycerol, urea, ammonia (NH3), carbon dioxide (CO2), hydrogen peroxide (H2O2), as well as metalloid such as boron and silicon (Tyerman et al, 2021). AQPs are divided into five subfamilies, including plasma intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs), and uncategorized X intrinsic proteins (XIPs) (Johanson et al, 2001). 35, 47, 41, 45, 43, 33, 47, and 35 AQPs have been identified in Arabidopsis (Johanson et al, 2001), Solanum lycopersicum (Reuscher et al, 2013), Phaseolus vulgaris (Ariani and Gepts, 2015), Manihot esculenta (Putpeerawit et al, 2017), Zea mays (Chaumont et al, 2001), Oryza sativa (Sakurai et al, 2005), banana (Hu et al, 2015), and watermelon (Zhou et al, 2019), respectively
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