Abstract
In this study, we report anti-inflammatory and antioxidant activities of two acylated isoscutellarein glucosides isolated from ethyl acetate extract of Stachys subnuda aerial part. 4'-O-methylisoscutellarein-7-O-2''-O-(6∙∙∙-O-acetyl-β-D-allopyranosyl)-β-D-glucopyranoside (SS1) and isoscutellarein-7-O-2''-O-(6∙∙∙-O-acetyl-β-D-allopyranosyl)-β-D-glucopyranoside (SS2) were isolated as major compounds from ethyl acetate extract (SSEA). Also, 2 hydroxycinnamic acid derivatives, and 5 isoscutellarein glucoside derivatives in the SSEA were identified using LC-MS/MS. SS1 with IC50 values of 2.35 and 1.98 'g/mL and SS2 with IC50 values 13.94 and 12.76 'g/mL showed fairly strong antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) radicals, respectively. SS1 and SS2 inhibited 5-lipoxygenase (5-LOX) activity with IC50 values of 47.23 and 41.60 'g/mL, respectively. The results demonstrated that SS1 and SS2 have significant anti-inflammatory and antioxidant potential. Acylated flavonoid glycosides have been first reported for Stachys subnuda. Also, the activities of SS1 and SS2 have been investigated for the first time in this study.
Highlights
The Stachys genus is one of the largest species of the Lamiaceae family and is represented by about 300 species worldwide.[1]
The aim of this study was to test anti-inflammatory and antioxidant activities of major compounds isolated from ethyl acetate extract of aerial parts of endemic Stachys subnuda Montbret & Aucher Ex Benth
It was evaluated anti-inflammatory and antioxidant activities of major compounds isolated from ethyl acetate extract of aerial parts of endemic Stachys subnuda
Summary
The Stachys genus is one of the largest species of the Lamiaceae family and is represented by about 300 species worldwide.[1]. In the studies on other Stachys species, it has revealed that secondary metabolites are generally iridoids, flavone glycosides, diterpenes and essential oils.[2,5,6,7] it has been reported that various extracts of Stachys species have antioxidant, anti-proliferative, anti-inflammatory, antiulcer, antinociceptive, antimicrobial activities.[8,9,10] There is no scientific information on chemical composition of Stachys subnuda ethyl acetate extract and anti-inflammatory and antioxidant activities of its active major compounds. The aim of this study was to test anti-inflammatory and antioxidant activities of major compounds isolated from ethyl acetate extract of aerial parts of endemic Stachys subnuda Montbret & Aucher Ex Benth
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