Abstract
We isolated and characterized a total of 12 microsatellite loci from the marbled salamander, Ambystoma opacum. Loci were screened in 24 individuals from a single location on the Savannah River Site in South Carolina. The number of alleles per locus ranged from 3 to 15, observed heterozygosity ranged from 0.167 to 0.917, and the probability of identity values ranged from 8.5 × 10−2 to 3.2 × 10−1. These new loci will provide tools for examining the genetic diversity, structure, and mating system of A. opacum populations.
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