Abstract
Coffea canephora is an industrial plant for the people of Tay Nguyen. The application of bio-fertilizer has been conducted in order to replace inorganic fertilizers because bio-fertilizer could support high yield and keep the healthy environment. In this research, one hundred bacterial strains were isolated from roots, leaves and berries of Coffea canephora, grown in Ea H’leo and Krong Nang districts, Dak Lak province. The morphological characteristics of almost colonies were round shape, raised or convex elevation, entire margin, pure white or milky white or yellow. Almost bacterial cells had rod shape, motile, Gram negative and all of them made pellicles in LGIP, NFb and BAz semi solid media after seventy-two hours of incubation. The results showed that strain L.R150-3 had the highest ability of N2-fixing with 0.289 mg/L of NH4+ concentration, while strain B.R157-2 had the highest ability of solubilizing insoluble phosphate with 4.30 mg P2O5/mL and strain L.R150-1 gave the highest amount of indole acetic acid at 19.206 μg/mL. Three strains L.R150-3, B.R157-2 and L.R150-1 were determined respectively as Kosakonia sp. L.R150-3, Burkholderia sp. strain Eb-6 (KJ865578.1) B.R157-2 and Enterobacter sp. L.L150-1.
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