Abstract
A gram-negative, budding, catalase negative, oxidase positive and non-motile bacterium (MBLW1T) with a complex endomembrane system has been isolated from a freshwater lake in southeast Queensland, Australia. Phylogeny based on 16S rRNA gene sequence analysis places the strain within the family Planctomycetaceae, related to Zavarzinella formosa (93.3 %), Telmatocola sphagniphila (93.3 %) and Gemmata obscuriglobus (91.9 %). Phenotypic and chemotaxonomic analysis demonstrates considerable differences to the type strains of the related genera. MBLW1T displays modest salt tolerance and grows optimally at pH values of 7.5–8.0 and at temperatures of 32–36 °C. Transmission electron microscopy analysis demonstrates the presence of a complex endomembrane system, however, without the typically condensed nucleoid structure found in related genera. The major fatty acids are 16 : 1 ω5c, 16 : 0 and 18 : 0. Based on discriminatory results from 16S rRNA gene sequence analysis, phenotypic, biochemical and chemotaxonomic analysis, MBLW1T should be considered as a new genus and species, for which the name Tuwongella immobilis gen. nov., sp. nov. is proposed. The type strain is MBLW1T (=CCUG 69661T=DSM 105045T).
Highlights
A gram-negative, budding, catalase negative, oxidase positive and non-motile bacterium (MBLW1T) with a complex endomembrane system has been isolated from a freshwater lake in southeast Queensland, Australia
The family Planctomycetaceae belongs to the phylum Planctomycetes within the domain Bacteria, members of which possess distinctive properties such as a complex endomembrane system, budding reproduction and the ability to perform endocytosis-like protein uptake [1, 2]
The family Planctomycetaceae is formed by the described genera Blastopirellula, Gemmata, Gimesia, Pirellula, Planctomicrobium, Planctopirus, Rhodopirellula, Rubinisphaera, Schlesneria, Telmatocola, Thermogutta and Zavarzinella [3,4,5,6,7,8,9,10,11]
Summary
Seeger et al, Int J Syst Evol Microbiol 2017;67:4923–4929 DOI 10.1099/ijsem.0.002271 Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes Christian Seeger,1 Margaret K. Butler,2 Benjamin Yee,1 Mayank Mahajan,1 John A. Fuerst3 and Siv G. E. Andersson1,*
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Systematic and Evolutionary Microbiology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
