Abstract
The turnover of sulphated glycosaminoglycans (GAG) in cultures of normal glial and malignant glioma cells was studied in pulse-chase experiments, using 35SO4 as a marker. The cell surface associated 35S-GAG of normal cultures had an initial half-life time of 10–14 h, followed by a second phase with a half-life of 40–90 h. After 48 h of chase, about 75% of the 35S-GAG was eliminated. The major turnover route was via degradation to inorganic 35SO4 (about 60%), but some 35S-GAG (about 40%) was transferred in apparently non-degraded form to the medium. The pericellular 35S-GAG of a malignant glioma cell line showed a somewhat faster turnover. Most of the glioma cell 35S-GAG was released to the medium rather than degraded. The cell density had little influence on the turnover of 35S-GAG in normal or malignant cell cultures. Substrate-attached material (SAM) from either cell type showed a low rate of turnover.
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