Turnip yellows virus and Soybean dwarf virus in Western Australia

Abstract

In Western Australia (WA) viruses belonging to the Luteoviridae have been identified infecting crops, including Barley yellow dwarf virus, Cereal yellow dwarf virus, Phasey bean mild yellows virus, Potato leaf roll virus, and Turnip yellows virus (TuYV). It is thought there are other viruses of the Luteoviridae in WA, however efforts to determine their presence have been greatly hindered by the very close serological relationship of many virus species within the Luteoviridae. Research on TuYV in Australia has predominantly been on canola (Brassica napus) but it has also been detected on other hosts. Between 2006 and 2014, virus isolates from a range of hosts and locations were obtained following serological testing using antisera for Beet western yellows virus (BWYV). Molecular characterisation using next generation sequencing (NGS) produced whole genome sequences of ten virus isolates with seven new TuYV and three Soybean dwarf virus (SbDV) genomes identified. In addition, biological studies of two TuYV isolates were done. We discuss the relationship of these ten new isolates within the Luteoviridae and determined BWYV not to be present in WA.