Abstract
A competitive binding radioimmunoassay (RIA) was developed utilizing 125I-labeled turkey β 2-microglobulin (β 2m), rabbit anti-turkey β 2m serum and polyethylene glycol for separation of bound and free antigen. The antibody-antigen reaction was demonstrated to be monospecific by immunofixation electrophoresis. Characteristic parameters of the RIA were: nonspecific binding, 5.5%; linear dose-response range, 0.24–64.0 ng turkey β 2m; dose-response correlation coefficient, −0.995; assay sensitivity, 0.52 ng; upper limit of precision, 40.4 ng. RIA analysis of adult turkey tissue and organ homogenates revealed highest amounts (> 10 μg turkey β 2 m/g) in liver and kidney and intermediate levels (2–10 μg/g) in lymphoid organ (thymus, spleen, caecal tonsil, bursa), skin and lung extracts. Erythrocytes and peripheral blood lymphocyte extracts contained 8.0 and 5.1 μg/10 9 cells respectively whereas all other tissues examined expressed < 2.0 μg/g. The β 2 m content in turkey serum was 192.5 μg/ml. The molecular weight distribution of turkey β 2m in serum determined by gel permeation chromatography consisted of a minor component of approximately 60,000 daltons and a major fraction eluting at a position characteristic of free β 2m. A cross-reaction between turkey and mammalian β 2ms was not observed.
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