Abstract
Advances in genetic transformation of turfgrass, like other major crop species, have been made due to the development of plant regeneration from embryogenic tissue cultures. Two conventional methods of gene delivery, direct DNA uptake by protoplasts and biolistic transformation have been used successfully to produce several species of transgenic forage grass and turfgrass. In transformation, antibiotics (hygromycin or G418), and herbicides (bialaphos, or phosphinothricin) are used to select transformed cell lines. Reporter gene ( gusA) is used to document the expression of a foreign gene in the transformants either transiently or stably. Several field test permits have been issued by USDAAPHIS for trials of transgenic creeping bentgrass. However, assessment of the potential environmental risks from transgenic turfgrass need to be made before commercial release.
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