Abstract
A series of rhodamine-coumarin conjugates with tunable linkers function as FRET based sensors for biological Al3+ imaging in living HeLa cells under fluorescence microscope. In methanol, all probes show chelation enhanced fluorescence (CHEF) assisted fluorescence resonance energy transfer (FRET) process. A remarkable change in FRET efficiency is observed with increasing water content of the media for a particular probe. DFT studies corroborate experimental observations.
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