Tuning alginate-bentonite microcapsule size and structure for the regulated release of P. putida Rs-198

Abstract

In this study, different sizes of microcapsules with alginate and bentonite as natural macromolecular materials were prepared to investigate the release property of Pseudomonas putida Rs-198. The characteristics of three microcapsules were evaluated by SEM, FTIR, TG-DSC, XRD and wall thickness. The sizes of three microcapsules (MA, MB, and MC) were 1270.50, 831.79 and 42.52 μm, respectively. First, the encapsulation efficiency of three MA, MB, and MC microcapsules were 82.20%, 90.41%, and 85.84%, respectively. Second, the contact angles of MA and MB samples were similar, while smaller microcapsules MC have higher contact angle (85.05°), indicating poor hydrophilia and decreasing the swelling degrees. Third, the release cumulant of Rs-198 and macromolecule BSA linear stage was fitted to self-established mathematic model. Results show that the microcapsule size had a considerably positive effect on release detail. The large microcapsule possessed strong leak-tightness for Rs-198 as a slow-release microbial agent. Furthermore, the porosity of microcapsules determined their swelling and release and may affect bacterial growth and survival. In conclusion, the Rs-198 microcapsule with different sizes will be pertinently selected based on the characteristics of agricultural production requirements.