Tunable enzymatic biodegradation of poly(butylene succinate): biobased coatings and self-degradable films

Abstract

Biodegradation of polyesters driven by enzymes is considered as one of the most effective way of degradation of these materials, as a way to control plastics accumulation in the environment. In this study, we present two different strategies to tune the enzymatic degradation of PBS films triggered by a lipase from Pseudomonas cepacia. Firstly, the kinetics of enzymatic degradation of PBS films was regulated by applying multilayer coats of polysaccharide alginate and chitosan (Alg/Chi) films. Secondly, self-degradable PBS films were prepared by embedding lipase-filled alginate particles. For comparison purposes, a detailed enzymatic degradation study of neat PBS films exposed to a lipase from P. cepacia in solution was made to determine the main experimental parameters influencing their degradation in solution. The results showed that an increase in enzyme concentration increased the degradation extent and rate of neat PBS films. At a fixed enzyme concentration, stirring of the solution containing the enzyme and the PBS also increased the biodegradation rate. In the case of the PBS films coated with a different number of Alg/Chi layers by spray-assisted LbL and subjected to enzymatic degradation experiments in solution, the extent of degradation was found to be dependent on the number of protective coating layers. Therefore, the Alg/Chi biobased coating constitutes an effective barrier to the diffusion of the lipase, thus proving its effectiveness in modulating the enzymatic activity as a function of coating thickness. In the case of self-degradable PBS containing lipase-embedded alginate beads (employed to protect the enzyme during high-temperature processing), only limited biodegradation was observed as the amount of encapsulated enzyme employed was too small. Nonetheless, these results are promising, as the enzymatic activity –indicative of the degradation capacity of the enzyme– determined for all these samples was about 2 orders of magnitude lower than that of previous assays.