Abstract
The present report deals with the synthesis of plant-mediated copper oxide nanoparticles (pm-CuO NPs) from Annona squamosa aqueous seed extract for effective antibacterial activity and the further utilization of this as a platform for the electrocatalytic determination of hydrogen peroxide (H2O2) for applications in the agricultural domain. The structural, optical and morphological characteristics of the synthesized pm-CuO NPs were analyzed by UV-Vis, XRD, FT-IR, AFM, SEM, TEM, HR-TEM and EDX. After this, pm-CuO NPs were preliminarily investigated for antibacterial activity on Gram-positive and Gram-negative bacterial strains, and further, their activity was validated for assessing their antibacterial efficiency on the Xanthomonas oryzae, a plant pathogenic bacteria strain, and the obtained results showed that pm-CuO NPs have potency as an effective antibacterial agent for the treatment of the bacterial blight of rice caused by X. oryzae in the rice crop, which reduces the rice crop productivity. Further, pm-CuO NPs were electrophoretically deposited onto an indium–tin–oxide (ITO) glass substrate and assessed for the electro-oxidation of H2O2 by cyclic voltammetry (CV), and from this it was proved that pm-CuO NPs had a very high electrochemical sensitivity of 49 μA μM−1 cm−2 towards H2O2 and a low detection limit of 574 μM, with these responses obtained under optimized experimental conditions. Thus, pm-CuO NPs also provide a potential sensing platform for electrochemical studies to detect H2O2 produced during plant stress surroundings to properly manage crops susceptible to oxidative damage by elevated H2O2 levels during stress.
Highlights
Over the past decade, there have been interdisciplinary elds of nanoscience and nanotechnology, interspersing material science, bionanosciences, and biotechnology
In this work here we hypothesized that the biogenic pm-CuO NPs have potential applications in the agricultural domain, and to prove this hypothesis, we demonstrated the electrochemical sensing response to H2O2, as it is very toxic to plants when produced in a higher concentration during plant stress conditions and it results in oxidative damage to plants and if not controlled it will lead to plant death
Biogenically fabricated CuO NPs were characterized for their optical, structural and morphological properties and further investigated for their applications in the agricultural domain by checking the pm-CuO NPs utility as an antibacterial agent for combating plant pathogenic bacterium and the utilization of pm-CuO NPs to fabricate a label-free sensor for the quantitative determination of H2O2 in plants for taking proper measures to help plants combat stress conditions
Summary
There have been interdisciplinary elds of nanoscience and nanotechnology, interspersing material science, bionanosciences, and biotechnology. The synthesis of metal oxide nanoparticles is an important research subject in modern physics due to their extraordinary capabilities in the elds of electronics, magnetics, optoelectronics, information storage and drug delivery.[1,2,3,4] Nanoparticles of different shapes and sizes are subject to great interest due to their possible. The plant-mediated synthesis of nanoparticles and their aspects have gained a lot of attention nowadays due to the unique properties exhibited by nanoparticles synthesized from this route.[14,15,16] biogenic metal/metal oxide-based nanoparticles have a wide range of utility in various elds, and in agriculture, they are widely used as an antimicrobial agent, for monitoring the plant stress condition and for the detection of effluents (pesticides, by-products in pesticides (hydrazine), etc.) produced from erroneous agricultural practices.[17,18,19]
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