Abstract
Purified anti-P815 IgG or F(ab 1) 2 from rabbits or sheep were measured for lytic capacity on 51Cr-labelled murine P815 tumour cells with complement from either rabbits, sheep, rats or guinea-pigs. Rabbit IgG caused lysis with all complement sources but sheep IgG was lytic only with rabbit and sheep complements. Rabbit F(ab 1) 2 fragments caused target cell lysis with rabbit and sheep complements but not with rat or guinea-pig; sheep (F(ab 1) 2 was lytic only with rabbit complement. Using EGTA to remove Ca 2+ from the assay system sheep and rabbit complements were variably activated via either the classical or alternate pathways, but rat and guinea-pig complements were activated only via the classical pathway. Some possible significances of these findings to serology are discussed.
Published Version
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