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Abstract
Tumour growth depends on neovascularisation and tumour cell proliferation. Factor VIII-related antigen (F-VIII RA) localises to vascular endothelium. Expression of proliferating cell nuclear antigen (PCNA) is correlated with cell proliferation. We investigated the correlation between the expression of these antigens and prognosis in gastric carcinoma. A total of 108 specimens resected from patients with gastric carcinoma were investigated by staining with monoclonal antibodies against F-VIII RA and PCNA. Microvessel count (MVC; the mean number of microvessels in the five areas of highest vascular density at 200 x magnification) and PCNA labelling index (PCNA LI; percentage of positive cells in more than 500 tumour cells) were determined. The results showed that prognosis was significantly worse in patients who had a tumour with a high MVC (16 or greater) or a high PCNA LI (42% or greater) than in those patients who had a tumour with a low MVC (less than 16) or a low PCNA LI (less than 42%). Furthermore, MVC was significantly associated with the risk of hepatic recurrence. In conclusion, both MVC and PCNA LI may be good prognostic indicators in patients with gastric carcinoma.
Highlights
We investigated the correlation between prognosis and angiogenesis and cell proliferation in gastric cancer as demonstrated by immunohistochemical staining of F-VIII RA and proliferating cell nuclear antigen (PCNA) respectively
PCNA labelling index (PCNA LI) ranged from 7.7% to 76.5% with a mean value of
Neither Microvessel count (MVC) nor PCNA LI was associated with patients' age and sex
Summary
Resected specimens from 108 patients with gastric carcinoma who underwent curative surgical resection at our institution were studied. No patient had received chemotherapy or radiation therapy before the surgery. All patients were followed up for at least 5 years after surgery and routinely studied by diagnostic imaging (computerised tomography or ultrasonography) once or twice a year. The type of recurrence was established by diagnostic imaging, serum level of tumour marker, cytology, biopsy or surgery, and was classified as hepatic recurrence, peritoneal recurrence or another type of recurrence. The relationship between MVC, PCNA LI and clinicopathological factors was examined by the Wilcoxon rank sum test. The relationship between MVC, PCNA LI, various clinicopathological factors and the mode of recurrence was examined by chi-square test or logistic regression analysis.
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