Abstract
Objective To explore the influence of PI3K/Akt signaling pathway on invasive and migratory ability of pancreatic cancer cell line PANC-1, and further investigate the molecule mechanism of tumor-associated macrophages’promotion in the occurrence and development of pancreatic cancer. Methods Mononuclear cells were isolated from peripheral blood of healthy adults by density gradient centrifugation, and treated with IL-4 to obtain M2 in vitro. Expression of PI3K and Akt was evaluated by quantitative Real-time polymerase chain reaction and Western blotting. The ability of cellular invasion and migration was assessed by transwell chamber assay and wound healing assay. Results To simulate pancreatic cancer microenvironment, we co-cultured pancreatic cancer PANC-1 cells and Ua or M2. The mRNA and protein levels of PI3K and Akt were remarkably increased analyzing by qRT-PCR and western blotting. In addition, transwell chamber assays and wound healing assays revealed that alternatively activated macrophages significantly promoted the invasion and migration of PANC-1 cells in 20 h. Conclusion Tumor-associated macrophages may promote invasion and migration of pancreatic cancer cells via PI3K/Akt signaling pathway. Key words: Pancreatic cancer; Tumor-associated macrophages; PI3K; Akt; Invasion and migration
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
