Abstract
Background Long interspersed element 1 (LINE-1) is a retroelement comprising about 17% of the human genome, encoding ORF1 with an RNA interaction domain and ORF2 with endonuclease and reverse transcriptase activities. Similar to retroviruses, LINE-1 replicates in the host via an RNA intermediate that is reverse transcribed and integrated into the host genome. LINE-1 retrotransposition has resulted in genetic diseases. In fact, LINE-1 insertion was recently found in several human tumors. However, the life cycle of LINE-1 is not fully understood. Therefore, we examined identification and characterization of host factors affected the retrotransposition of LINE-1.
Highlights
Long interspersed element 1 (LINE-1) is a retroelement comprising about 17% of the human genome, encoding ORF1 with an RNA interaction domain and ORF2 with endonuclease and reverse transcriptase activities
We found that MOV10 markedly inhibited the retrotransposition of LINE-1 as well as APOBEC3G/F
LINE-1 ORF1 colocalized with MOV10 or APOBEC3G in P-bodies
Summary
Long interspersed element 1 (LINE-1) is a retroelement comprising about 17% of the human genome, encoding ORF1 with an RNA interaction domain and ORF2 with endonuclease and reverse transcriptase activities. LINE-1 replicates in the host via an RNA intermediate that is reverse transcribed and integrated into the host genome. LINE-1 retrotransposition has resulted in genetic diseases. LINE-1 insertion was recently found in several human tumors. The life cycle of LINE-1 is not fully understood. We examined identification and characterization of host factors affected the retrotransposition of LINE-1
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