Abstract
BackgroundMicroRNAs (miRNAs) play crucial functions in the progression of ovarian cancer. MicroRNA-27b-5p (miR-27b-5p) has been identified as a cancer-associated miRNA. Nevertheless, the expression profile of miR-27b-5p and its functions in ovarian cancer are unexplored.MethodsqRT-PCR and western blot analysis were used to detect the levels of miR-27b-5p and C-X-C motif chemokine ligand 1 (CXCL1). The impact of miR-27b-5p on ovarian cancer cells proliferation, migration and invasion in vitro were investigated using Cell Counting Kit-8 (CCK8), wound healing and Transwell, respectively. The expression of matrix metalloprotein-2/9 (MMP-2/9) were measured using immunofluorescence staining. Bioinformatics and luciferase reporter analysis were used to predict the target of miR-27b-5p. The growth of ovarian cancer cells in vivo was evaluated using transplanted tumor model.ResultsHere, we demonstrated that miR-27b-5p was downregulated in ovarian carcinoma cells and clinical specimens. Higher expression of miR-27b-5p was associated with an unfavorable overall survival in patients with ovarian cancer. Upregulation of miR-27b-5p decreased the viability, migration ability and invasion capacity of SKOV3 and OVCAR3 cell. MiR-27b-5p also inhibited the growth of SKOV3 cell in nude mice. Additionally, we verified that CXCL1 was a target of miR-27b-5p in ovarian carcinoma cells. Restoring the expression of CXCL1 abolished the inhibitory impacts of miR-27b-5p in ovarian cancer carcinoma cells.ConclusionThis research revealed that miR-27b-5p restrained the progression of ovarian carcinoma possibly via targeting CXCL1.
Highlights
Ovarian carcinoma is still one of the most lethal gynecological malignancies
MiR-27b-5p is downregulated in ovarian carcinoma Firstly, we checked the expressions of miR-27b-5p in human ovarian carcinoma samples
Results indicated that miR-27b-5p was drastically downregulated in ovarian carcinoma cells compared with in human ovarian surface epithelial cell line, HOSEpiC (Fig. 1B)
Summary
Ovarian carcinoma is still one of the most lethal gynecological malignancies. Most deaths from ovarian cancer are attributed to distant metastasis. Metastasis, which is a multistep process, allows tumor cell diffuse from the primary sites to distant tissues [1, 2]. Increasing investigations have demonstrated that the loss of cancer. MiRNAs are a class of non-coding RNAs and modulate the expressions of target genes via binding to the 3′untraslated region (3′-UTR) of mRNAs [5]. Various miRNAs participate into the progression of malignant cancers [6,7,8]. MicroRNAs (miRNAs) play crucial functions in the progression of ovarian cancer. MicroRNA-27b-5p (miR-27b-5p) has been identified as a cancer-associated miRNA. The expression profile of miR-27b-5p and its functions in ovarian cancer are unexplored
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