Abstract
The abnormality of immune regulation plays a critical role in the pathogenesis of cancer; the underlying mechanism has not been fully understood yet. This study aims to investigate the role of cancer specific T helper (Th)2 response in the inhibition of colon cancer (Cca) cell growth. The results showed that with Cca cell (CT26 cell) extracts as an antigen, the Cca-extract specific Th2 response was induced in the Cca-bearing mice. The Cca mass size was significantly reduced, or radically disappeared (5 out of 10; or 50%); the survival rate was markedly improved in mice immunized with Cca-extract, but not in those immunized with another tumor cell (U87 cell) extracts or to bovine serum albumin. The immunization with Cca-extract also induced Cca cell apoptosis and converted the intra-Cca Tregs to T helper (Th) 9 cells. In conclusion, Cca-specific Th2 responses inhibit Cca growth in a mouse model via inducing Cca cell apoptosis and converting intra-Cca Tregs to Th9 cells.
Highlights
The abnormality of immune regulation plays a critical role in the pathogenesis of cancer; the underlying mechanism has not been fully understood yet
The results showed that cells from naïve mice showed less than 5% cell proliferation (Fig. 1A,F); in the presence of the colon cancer (Cca)-extract, 47.9% CD4+ T cell proliferation was detected in the Cca-bearing mice immunized with Cca-extract (Fig. 1B,F); cells from Cca-bearing mice immunized with U87-extract (Fig. 1C,F), or BSA (Fig. 1D,F), or treated with alum (Fig. 1E,F) alone showed 4.69%, 5.79% and 4.10%, respectively (p > 0 .05 as compared with Fig. 1A)
The results showed that the apoptosis was still induced in Cca cells (Fig. 3L,N), which indicates that the cell-cell contact is unnecessary in the induction of Cca cell apoptosis by the present experimental setting
Summary
The abnormality of immune regulation plays a critical role in the pathogenesis of cancer; the underlying mechanism has not been fully understood yet. This study aims to investigate the role of cancer specific T helper (Th)[2] response in the inhibition of colon cancer (Cca) cell growth. Cca-extract, but not in those immunized with another tumor cell (U87 cell) extracts or to bovine. The immunization with Cca-extract induced Cca cell apoptosis and converted the intra-Cca Tregs to T helper (Th) 9 cells. Cca-specific Th2 responses inhibit Cca growth in a mouse model via inducing Cca cell apoptosis and converting intra-Cca Tregs to Th9 cells. In specific circumstances, the cancer killer cells may be dysfunctional; such as the Rintratumor infiltrating regulatory T cells (Treg) are capable of suppressing most effector T cell activities to impair the anti-tumor mechanism in the body.
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