Abstract
BackgroundRenal cell carcinoma (RCC) is a common urological cancer. circular RNAs (circRNAs) is involved in the development of various types of cancers. However, the roles and underlying mechanisms of circRNAs in RCC are not fully elucidated. Herein, we aimed to examine the potential effect of circ_001287 on RCC progression.Materials and MethodsMicroarray-based gene expression profiling of RCC was initially employed in order to identify differentially expressed genes. Next, the expression of circ_001287 was examined, and the cell line with the highest circ_001287 expression was selected for subsequent investigation. The interaction among circ_001287, miR-144, and CEP55 was identified by conducting luciferase reporter assay, RNA-pull down, RIP, RT-qPCR and FISH. The effect of circ_001287 on proliferative, invasive and migratory capacities as well as tumorigenicity of transfected cells in mice was examined using gain- and loss-of-function experiments.Resultscirc_001287 and CEP55 were highly expressed while miR-144 was decreased in RCC tissues and cell lines. circ_001287 can up-regulate CEP55 by binding to miR-144, which resulted in increased proliferative, invasive and migratory capacities and tumor growth in vivo. In addition, down-regulation of miR-144 was also observed to promote these biological activities.ConclusionsOverall, these results elucidate a new mechanism for circ_001287 in RCC development and provide a potential therapeutic target for RCC patients.
Highlights
Renal cell carcinoma (RCC) is a common urological cancer. circular RNAs is involved in the development of various types of cancers
It was shown that in the GSE100666, GSE15641, GSE53757 and GSE71963 datasets, the expression of centrosomal protein 55 (CEP55) was higher seen in RCC tissues than that witnessed in adjacent normal tissues (Fig. 1e-h)
The bioinformatics analysis showed that upregulated circ_001287 and CEP55 in RCC and most importantly, miR-144 seems to involve in the regulation of these two genes
Summary
Renal cell carcinoma (RCC) is a common urological cancer. circular RNAs (circRNAs) is involved in the development of various types of cancers. Renal cell carcinoma (RCC) is a common urological cancer. The roles and underlying mechanisms of circRNAs in RCC are not fully elucidated. We aimed to examine the potential effect of circ_001287 on RCC progression. Renal cell carcinoma (RCC) ranks the third leading cause of death in humans suffering urological tumors [1]. RCC is a disorder that results from the renal epithelium and afflicts more than 90% of cancers in the kidney [3]. Patients suffering localized RCC can be treated with nephrectomy. The survival rate for patients with maligant RCC is reported to be extremely low [5]. The von Hippel Lindau (VHL) is a typical tumor suppressor gene, and most RCC have VHL gene abnormalities including VHL gene mutations, methylation and loss of heterozygosity [6]
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