TUMOR NECROSIS FACTOR RECEPTOR REGULATION OF BONE MARROW CELL APOPTOSIS DURING ENDOTOXIN-INDUCED SYSTEMIC INFLAMMATION

Abstract

Although inflammation-induced release of cells from the bone marrow (BM) is well established, less is known regarding inflammation-induced modulation of bone marrow cell numbers by apoptosis. The purpose of this study is to assess apoptosis of BM immature and mature myeloid cells and peripheral granulocytes, and to elucidate the role(s) of TNFR-p55 and TNFR-p75 as modulators of apoptosis in these cellular compartments in a mouse model of endotoxin-induced systemic inflammation. Gene knockout (p55(-/-), p75(-/-), and p55(-/-)/p75(-/-)), or wild-type (WT) mice were injected i.p. with saline (Sal) or LPS (4 microg/g) followed by collection of BM cells and peripheral blood after 24 h. Apoptosis was assessed by propidium iodide staining using two-color flow cytometry with differentiated granulocyte-specific Gr1-fluorescein isothiocyanate. Repeated-measures analysis of variance and Neuman-Keuls post hoc test were used for statistical analyses. After i.p. LPS, apoptosis was induced to the higher level in BM Gr1(-) cells than in BM Gr1(+) cells and was not induced in peripheral Gr1(+) cells. Depletion of cell numbers in both BM Gr1(-) and Gr1(+) subpopulations after LPS treatment was consistent with increase of the apoptotic cell percentages in the groups. LPS-induced apoptosis was significantly lower in Gr1(-) cells from the -p55(-/-)/LPS and p55(-/-)/p75(-/-)/LPS mice but not from p75(-/-)/LPS mice as compared with WT/LPS mice, whereas there was no difference in apoptosis of BM Gr1(+) and peripheral Gr1(+) cells among WT groups and knockout groups. Thus, apoptosis of myeloid cells during endotoxemia is minimized because these cells undergo differentiation, which in turn may be because of the attenuation of the proapoptotic effect of TNFR-p55 shown herein to occur with myeloid differentiation. In contrast, TNFR-p75 seems to play a minimal role in apoptosis induction in Gr1(-) myeloid cells during endotoxemia. One explanation for a decrease in BM cell numbers during endotoxemia may be via induction of apoptosis in immature myeloid cells.