Abstract
Nowadays there is growing evidence that some cytokines regulate biological functions of the mature mast cells. Therefore, we have studied whether TNF- α, the cytokine of multifunctional activities, could directly stimulate rat peritoneal mast cells to histamine secretion and whether it could modulate rat mast cell reactivity in anaphylactic (with ConA) and anaphylactoid (with compound 48/80) reactions. We have established that rat recombinant TNF- α does not activate rat mast cells to histamine release. However, TNF- α-treatment causes the decrease of spontaneous histamine release up to 85% (TNF- α concentration: 2×10 −9 M). Pretreatment of mast cells with TNF- α inhibits ConA-stimulate release of histamine with the percent release decreasing up to 33.7% of the control value (TNF- α concentration: 5×10 −9 M) and this decrease is statistically significant. Pretreatment of mast cells with TNF- α reduces compound 48/80-dependent histamine release as well and the percent release of histamine fell to 64.7% of the control value. We have concluded that TNF- α may play a significant role in regulation of mast cell secretory activity.
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