Abstract
Objective To study the protective effect of tumor necrosis factor (TNF)-α preconditioning on hepatic ischemia-reperfusion injury (IRI) and the underlying mechanisms,and the relationship between dose and effect.Methods Fourty healthy male Wistar rats were randomly divided into four groups:sham-operated (SO) group,ischemia-reperfusion (IR) group (total inflow occlusion for 30 min),low-dose TNF-α preconditioning (LTPC) group (intraperitoneal injection of 1 μg/kg TNF-α 30 min prior to IR) and high-dose TNF-α preconditioning (HTPC) group (intraperitoneal injection of 2 μg/kg TNF-α 30 min prior to IR).The samples of blood and hepatic tissue were taken after the experiment.The protein levels of nuclear factor-κB (NF-κB) p65 and B cell lymphoma/leukemia-2 (bcl-2) in the hepatic tissue were detected by immunohistochemistry.Results There was significant difference (P ＜ 0.05) between the IR group and the TPC groups in the levels of ALT and AST,the expression of NF-κB p65 and bcl-2,and apoptosis index in hepatic tissues.There was no significance difference (P ＞ 0.05) between the LTPC group and the HTPC group.Conclusion Low-dose TNF-α preconditioning decreased the intensity of hepatic IRI by inducing a decrease in the NF-κB p65 expression and an increase in the bcl-2 expression. Key words: Liver ischemia; Reperfusion injury; Tumor necrosis factor-α; Preconditioning
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