Tumor necrosis factor-alpha in peripical tissue exudates of teeth with apical periodontitis.

Sonja Pezelj-Ribarić,Jelena Prpić,Ivana Miletić,Karolina Magašic,Zoran Karlović

doi:10.1155/2007/69416

Sonja Pezelj-Ribarić, Jelena Prpić + Show 3 more

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https://doi.org/10.1155/2007/69416

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Abstract

Aim. The aim of this study was to determine tumor necrosis factor-alpha (TNF-) levels in periapical exudates and to evaluate their relationship with radiological findings. Methodology. Periapical exudates were collected from root canals of 60 single-rooted teeth using absorbent paper points. TNF- levels were determined by enzyme-linked immunosorbent assays. The samples were divided into three groups according to the periapical radiolucent area. Results. Nonparametric Kruskal-Wallis test revealed significant differences between TNF- concentrations in control group (40, , 15 pg/mL) and group with larger radiolucent areas (2365, , 95 pg/mL), as well as between control and canals with small radiolucent areas (507, , 97) (). Conclusions. The levels of TNF- increase significantly in teeth with periapical pathosis, from smaller to bigger lesions. This research and its results have shown that objective analysis of the TNF- levels enables establishment of a relationship between different concentrations of TNF- and different radiological changes.

Highlights

Periapicalpathoses of pulpal origin develop in response to microbial irritants in the root canal systems
The present study demonstrated the presence of TNF-α in all periapical exudate samples
Differences in concentrations of TNF-α between control group (40, 57 ± 28, 15 pg/mL), the group with large radiolucent areas (2365, 79 ± 582, 95 pg/mL), as well as between control and the canals associated with small radiolucent areas (507, 66 ± 278, 97) were statistically significant (P < .05)

Summary

Introduction

Periapicalpathoses of pulpal origin develop in response to microbial irritants in the root canal systems. Irritants may be mechanical or chemical, but are most often bacterial The interaction between these irritants and host defensive cells results in release of numerous mediators that, through the root canal system, are capable of initiating immunologic reactions in periapical tissues resulting in the formation on inflammatory periapical lesions. These reactions include immune response mediated by cells, through the actions of T lymphocytes and cytokines, and hummoral immune response mediated by antibodies, activated by B-lymphocyte products [2]

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