Abstract
BackgroundLiver transplantation is an important treatment option for patients with liver-originated tumors including biliary tract carcinomas (BTCs). Post-transplant tumor recurrence remains a limiting factor for long-term survival. The mammalian target of rapamycin-targeting immunosuppressive drug rapamycin could be helpful in lowering BTC recurrence rates. Therein, we investigated the antiproliferative effect of rapamycin on BTC cells and compared it with standard immunosuppressants.MethodsWe investigated two human BTC cell lines. We performed cell cycle and proliferation analyses after treatment with different doses of rapamycin and the standard immunosuppressants, cyclosporine A and tacrolimus.ResultsRapamycin inhibited the growth of two BTC cell lines in vitro. By contrast, an increase in cell growth was observed among the cells treated with the standard immunosuppressants.ConclusionsThese results support the hypothesis that rapamycin inhibits BTC cell proliferation and thus might be the preferred immunosuppressant for patients after a liver transplantation because of BTC.
Highlights
Liver transplantation is an important treatment option for patients with liver-originated tumors including biliary tract carcinomas (BTCs)
We demonstrate an antiproliferative effect of different doses of rapamycin on BTC cells, taking into account the established concentrations of rapamycin generally used for in vitro and in vivo studies
The low differentiated BTC cell line EGI-1 showed a homogenous inhibition of the division index in the rapamycin group
Summary
Liver transplantation is an important treatment option for patients with liver-originated tumors including biliary tract carcinomas (BTCs). Therein, we investigated the antiproliferative effect of rapamycin on BTC cells and compared it with standard immunosuppressants. Results: Rapamycin inhibited the growth of two BTC cell lines in vitro. Conclusions: These results support the hypothesis that rapamycin inhibits BTC cell proliferation and might be the preferred immunosuppressant for patients after a liver transplantation because of BTC. Liver transplantation (LT) is a therapeutic option for treatment of malignant liver tumors, including extrahepatic BTC. It is a treatment modality, besides liver resection, that offers a curative effect [5,6,7,8,9].
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