Abstract
The effects of tumorigenesis and tumor growth on the non-involved organs remain poorly understood although many research efforts have already been made for understanding the metabolic phenotypes of various tumors. To better the situation, we systematically analyzed the metabolic phenotypes of multiple non-involved mouse organ tissues (heart, liver, spleen, lung and kidney) in an A549 lung cancer xenograft model at two different tumor-growth stages using the NMR-based metabonomics approaches. We found that tumor growth caused significant metabonomic changes in multiple non-involved organ tissues involving numerous metabolic pathways, including glycolysis, TCA cycle and metabolisms of amino acids, fatty acids, choline and nucleic acids. Amongst these, the common effects are enhanced glycolysis and nucleoside/nucleotide metabolisms. These findings provided essential biochemistry information about the effects of tumor growth on the non-involved organs.
Highlights
It is conceivable that tumorigenesis and tumor growth at a specific organ sites demand more energy and nutrition that can be mobilized from other non-involved organs
On day 39 post inoculation (d39PI), the tumor sizes (n = 10) were significantly larger than that on day 6 post inoculation (d6PI) reaching about 10 mm in diameter with an average weight of about 0.48 g (Fig. 1)
Growth of solid tumors often accompanies with induced angiogenesis when tumor size reaches about 1–2 mm in diameter so as to meet the increased demands for energy, nutrition and other essential supplies such as hormones, growth factors and proteolytic enzymes for further growth and metastatic dissemination[18,19,20]
Summary
It is conceivable that tumorigenesis and tumor growth at a specific organ sites demand more energy and nutrition that can be mobilized from other non-involved organs. The effects of tumorigenesis and tumor growth on the biochemistry of other non-involved organs remain largely unknown. Such information is conceivably important for understanding the effects of tumors on patients as a whole and the therapeutic effects of effective treatments. By using the NMR-based metabonomics approaches in this study, we comprehensively analyzed the metabonomic phenotypes of multiple organs (heart, liver, spleen, lung, and kidney) for an A549 lung cancer xenograft mouse model at two tumor growth stages with tumor diameters of about 2 and 10 millimeters. The objective of this work is to define the effects of lung tumor growth on metabolism of the organs which are not directly involved prior to metastasis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
