Abstract
Increased MMP-1 expression in various tumor cells is significantly correlated with cancer progression. Enhanced secreted and intracellular level of MMP-1 is found in breast cancer cell line MDA-MB-231 in presence of ECM glycoprotein fibronectin. To extrapolate this study into in vivo system, we observed the expression of MMP-1, fibronectin and α5β1 integrin, which is the receptor for fibronectin, in breast cancer tissue samples. Expression of active form of MMP-1 was increased in tumor samples compared with the non-tumor counterpart. In some samples pro-MMP-1 was decreased but active form was increased in tumor part. The difference was more prominent in advanced stage tumor. ELISA showed appreciable increase in expression of α5 and β1integrins in tumor tissue in comparison to the non tumor counterpart in case of advanced stage tumor. Though there is no appreciable difference in fibronectin concentration, enhanced α5, β1 integrin expression may mediate enhanced cell-ECM interaction to upregulate MMP-1 in tumor samples compared with the matched control.
Highlights
Breast cancer is the most commonly occurring cancer in women, comprising almost one third of all malignan-How to cite this paper: Pal, S., Ganguly, K.K., Mandal, S., Biswas, J. and Chatterjee, A. (2015) Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer
We have studied MMP-2 [6] and MMP-9 [7] in relation to breast cancer
Western blot analysis showed that the expression of active form of MMP-1 was increased in tumor samples compared to the non-tumor control
Summary
Breast cancer is the most commonly occurring cancer in women, comprising almost one third of all malignan-How to cite this paper: Pal, S., Ganguly, K.K., Mandal, S., Biswas, J. and Chatterjee, A. (2015) Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer. (2015) Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer. The incidence of breast cancer has increased globally over the last several decades [2], the greatest increase has been in Asian countries [3]. Patients are about one decade younger in developing countries than their counterparts in the developed nations. In India, breast cancer is the commonest cancer among women in urban registries of Delhi, Mumbai, Ahmedabad, Kolkata, and Trivandrum where it constitutes >30% of all cancers in females [3]. There is a significant difference in the survival rates in developed and developing countries mainly because of a lack of screening and early detection programmes, insufficient information regarding possible cancer markers in that ethnicity and inadequate resources for treatment [3]. Studies on tissue samples found several MMPs to be associated with breast cancer development and tumor progression [5]. In the present communication we have dealt with MMP-1
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