Abstract
Sera from BALB/c mice bearing plasmacytomas were studied for the possible presence of soluble tumor-associated antigen (TAA) and anti-TAA antibodies. The antibodies were detected by passive hemagglutination, sheep red blood cells being sensitized by a soluble tumor extract as a source of antigen. TAA was detected by inhibition of the hemagglutination of a syngeneic antitumor immune serum. Sera were hemagglutinating (antibody activity), inhibitory (antigen activity), or neutral. A neutral pool of serum was submitted to exclusion chromatography, and the fraction containing the Ig 7S split with acid into 2 fractions: The one of high molecular weight (A) was hemagglutinating (antibody?); the other of low molecular weight (B) was inhibitory (antigen ?). The mixture A+B was neither hemagglutinating nor inhibitory. The peak of lowest molecular weight (albumin fraction) contained free antige.
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