Abstract
Previous studies have demonstrated that taurine-upregulated gene 1 (TUG1) was aberrantly expressed and involved in multiple types of cancer; however, the expression profile and potential role of TUG1 in prostate cancer (PCa) remains unclear. The aim of the present study was to evaluate the expression and function of TUG1 in PCa. In the present study, we analyzed TUG1 expression levels of PCa patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of TUG1 by RNAi was performed to explore its roles in cell proliferation, migration, and invasion. Here we report, for the first time, that TUG1 promotes tumor cell migration, invasion, and proliferation in PCa by working in key aspects of biological behaviors. TUG1 could negatively regulate the expression of miR-26a in PCa cells. The bioinformatics prediction revealed putative miR-26a-binding sites within TUG1 transcripts. In conclusion, our study suggests that long non-coding RNA (lncRNA) TUG1 acts as a functional oncogene in PCa development.
Highlights
Prostate cancer (PCa) is the second most frequently diagnosed cancer in men that leads to second or third cancer-related deaths worldwide [1]
In order to evaluate whether PCa cells would be sensitive to Taurine-upregulated gene 1 (TUG1) blockade, we showed that TUG1 is overexpressed in PCa, that TUG1 inhibition increased cell apoptosis, that TUG1 knockdown in PCa cells resulted in a significant decrease in cell growth, migration, and invasion
TUG1 was highly expressed in diverse PCa cell lines, including LNCaP, DU145, PC3, and 22Rv1, as compared with that in an immortalized non-tumorigenic human prostate epithelial cell line RWPE1 cells, a finding confirmed by quantitative real-time PCR (qRT-PCR) assays (P
Summary
Prostate cancer (PCa) is the second most frequently diagnosed cancer in men that leads to second or third cancer-related deaths worldwide [1]. Metastasis and invasion are the main causes of these lethal consequences of PCa [3]. Deciphering the mechanism of invasive and metastatic behavior are of great importance for early diagnosis and therapy of PCa. Long non-coding RNAs (lncRNAs) are important new members of the family of ncRNAs with limited or no protein-coding capacity [4,5]. Cumulative evidence is emerging that lncRNAs impact the biological functions of many different cancer types, including PCa [6,7,8]. Recent studies have shown that the biological behavior of the tumor could be regulated by specific lncRNA factors [9]. Limited data are available for the molecular mechanisms of TUG1 in PCa, largely due to a lack of specific investigation. In order to evaluate whether PCa cells would be sensitive to TUG1 blockade, we showed that TUG1 is overexpressed in PCa, that TUG1 inhibition increased cell apoptosis, that TUG1 knockdown in PCa cells resulted in a significant decrease in cell growth, migration, and invasion
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