Abstract
High-performance liquid chromatography of ergot alkaloid. A method for the analysis of ergot alkaloids has been developed by high-performance liquid chromatography. A silica column was used for preparation of ergotinine and ergotoxine from ergot. Separation of the individual ergot alkaloids was successfully accomplished on a column of Hitachi Gel No. 3011-O (porous polystyrene modified by hydroxymethyl) with an eluent of n-hexane—ethanol—triethylamine (70:30:0.5, v/v). The method was applied to the analysis of α-ergocryptinine, ergocorninine, ergocristinine, α-ergocryptine, ergocornine and ergocristine in ergot and dihydroergocryptine (α + β), dihydroergocornine and dihydroergocristine in pharmaceutical tablets.
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