Étude de l'influence de quelques composés chimiques sur le contact parenchyme palissadique – parenchyme lacuneux : répercussion sur la morphogenèse du limbe de Manihot esculenta Crantz

Abstract

To test the involvement of (i) the cell wall, (ii) the contact between cell wall and plasmalemma, (iii) the plasmalemma, and (iv) the cytoskeleton, in both (i) the regulation of the mesophyllian mitotic activity and (ii) the establishment of the laminar organization, various chemicals were microinjected into the apoplasm lying between the spongy and palisade parenchymas. Chemicals modifying cell wall structure did not disturb laminar morphogenesis. Alteration of the cytoskeleton did not change laminar mitotic pattern. However, plasmalemma disturbance by chemicals (detergents, KCl, urea, ionophore A 23187) added from the middle of the 2nd to the 5th plastochron resulted in (i) the development of organoid enations, thus showing an alteration in the regulation of the mitotic activity, and (ii) the formation of dark spots, probably owing to the thickening of the palisade parenchyma, thus showing a transient alteration of the laminar mitotic pattern.