Étude d'une souche de Drosophila melanogaster non permissive au togavirus sindbis: Caractéristiques de la souche et déterminisme génétique du phénomène

Abstract

Summary Sindbis virus had been shown to multiply well in D. melanogaster , a non-natural host. In order to investigate thoroughly the relations between this virus and this host, we sought drosophila strains genetically refractory to Sindbis virus. A non-permissive (NP) strain was studied. The viral multiplication defect did not occur at the virions adsorption-penetration step in NP-flies cells; the block was located later in the viral cycle. The NP character followed a maternal inheritance pattern; the maintenance of the responsible cytoplasmic agent was not chromosome-linked; so, this strain could not be denominated «refractory. This phenomenon was not specific of Sindbis virus since it had been observed for two other togavirus, Semliki Forest and Getah viruses, but not for two rhabdoviruses. Presently, investigations were in progress to further characterize this hereditary cytoplasmic factor, in NP-drosophila cell lines in vitro .