Tubulysin Production by the Dead Cells of Archangium gephyra KYC5002.

Abstract

Archangium gephyra KYC5002 produces tubulysins during the death phase. In this study, we aimed to determine whether dead cells produce tubulysins. Cells were cultured for three daysuntilthe verge of the death phase, disrupted via ultrasonication, incubated for 2h, and examined for tubulysin production. Non-disrupted cells produced 0.14mg/L of tubulysin A and 0.11mg/L of tubulysin B. Notably, tubulysin A production was increased by 4.4-fold to 0.62mg/L and that of tubulysin B was increased by 6.7-fold to 0.74mg/L in the disrupted cells. The same increase in tubulysin production was observed when the cells were killed by adding hydrogen peroxide. However, when the enzymes were inactivated via heat treatment of the cultures at 65°C for 30min, no significant increase in tubulysin production due to cell death was observed. Reverse transcription-quantitative polymerase chain reaction analysis of tubB mRNA revealed that the expression levels of tubulysin biosynthetic enzyme genes increased during the death phase compared to those during the vegetative growth phase. Our findings suggest that A. gephyra produces biosynthetic enzymes and subsequently uses them for tubulysin production in the cell death phase or during cell lysis by predators.