Abstract
BackgroundTubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition.MethodsPlacental tissue from normotensive (n = 25) and pre-eclamptic (n = 25) pregnancies were used to study the differentially expressed proteins by two-dimensional gel electrophoresis and TINAGL1 protein was validated with Western blotting.ResultsA total of 55 protein spots were differentially expressed (fold change >1.5, p < 0.05), of which 27 were upregulated and 28 were downregulated in the pre-eclamptic placenta. TINAGL1 was found to be downregulated in pre-eclamptic compared to normotensive pregnant women.ConclusionThis is the first study reporting TINAGL1 to be present in human placenta and differentially expressed in pre-eclamptic condition. The functional role of TINAGL1 in association to human pregnancy needs to be explored further.
Highlights
Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction
TINAGL1 is similar to tubulointerstitial nephritis antigen (TINAGL) in its protein domain composition, i.e. two EGF-like domains and a proteolytically inactive cathepsin B-like domain [1]
We investigated the placental proteome of preeclamptic women using proteomic techniques and found expression of TINAGL1 in the placenta
Summary
Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition. Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1) known as Lipocalin 7 (LCN7) or adrenocortical zonation factor-1 (AZ1) or tubulointerstitial nephritis antigen related protein (TIN-ag-RP) is a matricellular protein. The origin of PE remains enigmatic, the primary underlying cause is the abnormal placentation due to inefficient trophoblast invasion into the spiral artery. The reduced spiral artery remodeling leads to decreased blood flow to the placenta, making it more hypoxic. We investigated the placental proteome of preeclamptic women using proteomic techniques and found expression of TINAGL1 in the placenta
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